Fatty Acid Methyl Esters

Quantitative analysis of fat composition is extremely challenging. First of all it is necessary to apply highly toxic chemicals and large solvent volumes. As a result, the procedure is very tedious and virtually impossible to carry out in a non-laboratory environment.

We have developed a dedicated platform that is able to deal with the entire preparation procedure from fat to fatty acid methyl ester. The system uses the procedures described in AOCS Ce 2-66, which is based on triglyceride hydrolysis and reaction with BF3. After methylation, FAMEs are extracted in-vial with heptane and washed with NaCl/water. Some typical results are depicted in Table 1.

Table 1. Manual vs automated results.
Palm oil
  C12:0 C16:0 C18:0 C18:1 C18:2
Manual 0.40 35.0 3.62 46.1 12.5
Automated 0.40 35.1 3.56 46.1 12.4
20 samples          
%RSD man. 0.02 0.15 0.05 0.18 0.07
%RSD auto. 0.03 0.14 0.04 0.19 0.07

This system is available both as an off-line or as an on-line solution.

Oil and Fat Seminar

The FAME PAL series, developed by SampleQ some years ago, is our proven solution for automatic derivatization of fatty acids to fatty acids methyl esters.

Based on this experience, our team has co-created a similar solution for the analysis of 3-MCPD and glycidyl esters in edible oils in collaboration with our customers in the Netherlands.

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